Submerged macrophyte N. minor All. was collected from a clearwater
pond in a suburb of Wuhu City, Anhui Province, China. The
young sprouts (shoots and roots) of N. minor were cleaned in running
water to remove debris and washed in sterile water for three times,
and immersed in 10% sodium–hypochloride solution for 10 s and
then washed in sterile water three times again
[14].