2.4. Water holding capacity
Approximately 1 g of banana flour was weighed into pre-weighed 15 mL centrifuged tubes. To each sample, 10 mL of distilled water was added and mixed for 2 min. Samples were thoroughly wetted and allowed to stand at room temperature for 30 min after which they were centrifuged at 3000 rpm for 20 min. The resulting supernatant was decanted and the centrifuge tube containing sediment was weighed. Water holding capacity; gram of water per gram of protein was calculated