2.5.1. Instruments
For obtaining the profiles of phenolic compounds or protein/
peptide in PPEs and fPEM extracts, separations of compounds were
achieved with a Luna C18 (2) column (250 mm 4.6 mm, 5 lm,
Phenomenex, Torrance, CA, USA) with gradient elution programs,
using a Shimadzu LC-10AD HPLC (Tokyo, Japan) equipped with a
ternary pump delivery system and a UV–Vis detector.