Working in a laminar flow
cabinet, the medium was poured into 90mm sterile plastic
petri dishes, to a thickness of 2–3mm, and allowed to set.
The centre of each test plate was subsequently inoculated
with a 5mm size plug of 7–10-day-old cultures, for each of
the pathogens separately, and incubated for eight days at 25
± 2°C (March et al. 1991) in a growth cabinet. Radial
mycelial growth was determined after eight days by calculating
the mean of two perpendicular colony diameters on
each replicate.