Vials with 60μM of ZEA at distinct moisture levels (dehydrated and in water) were irradiated with 0, 2.0 and 10.0 kGy doses. ZEA levels were
determined by HPLC with fluorescence detection. Cytotoxicity of ZEA was
assessed in Hep G2 cells using a battery of assays covering different modes
of action including alterations of metabolic activity (AlamarBlue assay),
plasma membrane integrity (CFDA-AM assay) and lysosomal function (NRU
assay). The estrogenicity was assessed in HeLa 9903 cells, measuring
luciferase activity.