. The supernatant was passed through Diaion HP-20 and eluted with water and MeOH, successively. The MeOH eluate was then subjected to ODS eluted with starting from 40% aq.
MeOH to MeOH, gradiently. The 70% eluate provided esculeoside A (1, 440 mg). Similarly, from the pink type tomato [L. esculentum (Momotaro)] (7.53 kg), esculeoside A (1) was obtained in a yield of 311 mg.