region of the dominant arm. The blood was allowed to clot for 30
minutes at room temperature and centrifuged for 10 minutes to
obtain serum. After separation, all serum samples were stored at
–20°C until analysis for CK and LDH activity [21]. Serum CK activity
was determined spectrophotometrically by using a test kit CK (NAC
act.) kit (Mod. IFCC Method) CORAL, Goa, INDIA and LDH (P-L) kit
(Mod. IFCC Method) CORAL, Goa, INDIA.