in the extracts was completely removed by using a rotary evaporator
to obtain a semi-solid mass and the yield was calculated
based on the weight of the dried plants. A portion of the
resulting crude extract was fractioned by a separation funnel
using n-hexane, ethyl acetate, and water as solvents. Eluates
were collected in 1 L Erlenmeyer flasks and each fraction was
subjected to evaporation under reduced pressure in a rotary
evaporator. Fractions were stored at 4 C until assayed.