Cultures of Leishmania braziliensis and Leishmania infantum were obtained from Instituto de Investigaciones en Ciencias de la Salud,Asunción, Paraguay-IICS and identified by isoenzyme analysis.Tests for inhibition of promastigote forms were performed using a strain of L. braziliensis (MHOM/BR/75/M2903) and L. infantum (MCAN/ES/92/BCN83), grown at 28 C for 72 h in Schneider’s Drosophila medium supplemented with 20% FBS.