The marine algae N. oculata SCCAP K-1281was purchased from
The Scandinavian Culture Collection of Algae and Protozoa at the
University of Copenhagen. Artificial seawater (SW) was prepared
by aquarium salt (Red Sea’s Coral Pro Salt). In order to reach
required salinity, the amount of aquarium salt dissolved in a certain
volume of MilliQ water. Salinity was checked with Atago
Master-S/Mill M series refractometer. L1 medium was used in test
tubes with 20 mL and in conical flasks with 200 mL (in 500 mL
flasks) medium cultures to cultivate the alga specie. General experimental
conditions for 200 mL cultures, unless specified otherwise,
included incubation in 25 ppt sterilized SW at 25 ± 1 C under continuous
illumination of 50 lmol photons m2
s1
. All cultures were
stationary without aeration. To prevent sedimentation, flasks were
shaken by hand 2–3 times daily. Other kinds of growth medium,
Walne’s medium and enriched SW, were also tested for cultivation
of N. oculata species for 200 mL cultures. The enriched seawater
was artificial seawater supplemented with additional basic elements
containing 300 mg L1 urea, 45 mg L1 NaH2PO42H2O,
190 mg L1 NaSiO35H2O, 70 mg L1 Na2EDTA and 6 mg L1 FeCl3.
2.2. M
The marine algae N. oculata SCCAP K-1281was purchased fromThe Scandinavian Culture Collection of Algae and Protozoa at theUniversity of Copenhagen. Artificial seawater (SW) was preparedby aquarium salt (Red Sea’s Coral Pro Salt). In order to reachrequired salinity, the amount of aquarium salt dissolved in a certainvolume of MilliQ water. Salinity was checked with AtagoMaster-S/Mill M series refractometer. L1 medium was used in testtubes with 20 mL and in conical flasks with 200 mL (in 500 mLflasks) medium cultures to cultivate the alga specie. General experimentalconditions for 200 mL cultures, unless specified otherwise,included incubation in 25 ppt sterilized SW at 25 ± 1 C under continuousillumination of 50 lmol photons m2s1. All cultures werestationary without aeration. To prevent sedimentation, flasks wereshaken by hand 2–3 times daily. Other kinds of growth medium,Walne’s medium and enriched SW, were also tested for cultivationof N. oculata species for 200 mL cultures. The enriched seawaterwas artificial seawater supplemented with additional basic elementscontaining 300 mg L1 urea, 45 mg L1 NaH2PO42H2O,190 mg L1 NaSiO35H2O, 70 mg L1 Na2EDTA and 6 mg L1 FeCl3.2.2. M
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