The algae Botryococcus braunii, one of the potential renewable resources for production of liquid hydro-carbons, was used for testing the effect of selected herbicides on algal growth and hydrocarbon content.
Twenty-two herbicides representing 14 modes of action were assayed. Accelerated solvent extraction
(ASE) was used to extract hydrocarbon from algae and the results showed that ASE was an efficient
method for algal hydrocarbon extraction at 50
◦
C extraction temperature, 10.3 MPa of pressure and 1
static cycle using n-hexane as extraction solvent. The photosystem II inhibitor diuron was the most toxic
herbicide for algal growth, but hydrocarbon content increased from 34.9 to 42.4% of dry biomass in
the presence of a 0.1 mg/L concentration of diuron. The photosystem I inhibitor diquat was inhibitory
to growth of B. braunii, but hydrocarbon content increased to 43.3% of dry biomass when treated with
5 mg/L diquat. S-metalochlor, a mitosis inhibitor, reduced both algal growth and hydrocarbon content.
Hydrocarbon content decreased to 16.4, 14.0 and 5.5% of dry biomass, respectively when treated with 0.1,
1, and 5 mg/L S-metalochlor. The carotenoid biosynthesis inhibitor fluridone did not affect algal growth
at 0.1 mg/L, but decreased hydrocarbon content from 34.9 to 13.2%. The fatty acid and lipid biosynthesis
inhibitor thiobencarb had no effect on algal growth or hydrocarbon content at 0.1 mg/L, but it inhibited
algal growth and decreased hydrocarbon content from 34.9 to 7.8% at 1 mg/L. The oxidative phosphoryla-tion uncoupler dinoterb at 0.1 mg/L reduced algal biomass by 52.8% and decreased hydrocarbon content
from 34.9 to 30.0%. The use of B. braunii as a source of fuel will undoubtedly require the use of an out-door open pond system. These results will be useful for improving algal oil production while developing
management systems to control invasive algal species in outdoor open pond systems ultimately keeping
the oil producing algae pure.
© 2012 Elsevier B.V. All rights reserved