Murugan (2008) that showed that polyphenoloxidase enzyme from
acerola loses stability at temperatures above 75 C. The aforementioned
authors found that a heat treatment at 85 C for 3 min
reduces the enzyme activity to values close to 10%.
The samples were heated at voltages determined by a factorial
design. When the sample reached the desired temperature, the
voltage was reduced of approximately 50% to maintain the
temperature constant during 3 min. After this time, the water bath
was turned on and cool water passed through the water jacket of
the cell.