2.4. Cell culture
HT29 (human colon adenocarcinoma) cells from ATCC collection
were grown in McCoy's medium supplemented with L-glutamine
(2 mol/L), sodium pyruvate (200 g/L), fetal bovine serum (100 mL/L)
and antibiotics (100 U/mL penicillin and 100 g/L streptomycin). The
cells were grown at 37 C in humidified atmosphere containing 5%
CO2 in the Smart cell incubator (Heal Force)