been identified as S. aureus according to phenotype, but on
the basis of multi-locus sequence typing (MLST) and a
single genome sequence, the lineages are allied to but
significantly diverged from S. aureus. We describe here
investigations including the use of whole-genome sequence
analysis to justify classification as three separate species, S.
aureus, and two novel species of the genus Staphylococcus.
Strain MSHR1132T was isolated from blood cultures of an
Indigenous patient from Darwin, Australia (Holt et al.,
2011); and strain FSA084T was isolated from the nares of a
red-tailed monkey (Cercopithecus ascanius) from Gabon,
Africa (Schaumburg et al., 2012). Both strains grew on
tryptone soy agar (TSA) at 37 uC with large, round, smooth
colonies similar to typical S. aureus. Colonies of FSA084