Briefly, the catalytic activity of the arginase reactions was stopped by transferring 10 ll of reaction mixture into 750 ll of reagent A (20 mM phosphate buffer, pH 7, containing 60 mM salicylate, 1 mM sodium nitroprusside and >500 IU of urease).
Briefly, the catalytic activity of thearginase reactions was stopped by transferring 10 ll of reactionmixture into 750 ll of reagent A (20 mM phosphate buffer, pH 7,containing 60 mM salicylate, 1 mM sodium nitroprusside and>500 IU of urease).