to completion at 2 L of volume A 25 % solution was diluted to 4 % and Pipetted to adjust the pH to be in between pH 6.3 to pH 6.5 the obtained solution was kept at room temperature until use two kinds of water were used to prepare nano-chitosan solution in this experiment namely distilled water and clean water taken from a well The purpose was to see the difference in nano-chitosan strength when preserving fresh fish between the solution prepared with water produced in the laboratory scale and the one prepared from water used for ice blocks production The latter water source located in Latuhalat neardy the fishermen landing area was used to imitate as closely as possible the in situ condition of local fishermen preparation prior to fishing trips