The aim of thisstudy was to detect and quantify the amount of AFM1 in raw cow's milk and AFB1 in dairy feed samples in the Greater Addis Ababa milk shed using a value chain approach. For this purpose, 100 milk samples from dairy farmers and ten milk samples from milk traders were collected. In addition, 114 feed samples from dairy farmers and 42 feed samples from feed producers, processors and traders were collected