Acute RQs for fish and invertebrates were calculated by dividing the peak PECs by acute Predicted No Effect Concentrations (PNECs), while chronic RQs for algae, invertebrates, and fish were calculated by dividing the calculated 3-day, 21-day and 28-day TWAECs, respectively,by their respective chronic PNECs. Acute PNECs for invertebrates and fish were calculated by dividing the acute EC50 or LC50 values by an assessment factor of 100.