The shrimp were maintained in six replicate aquaria (40 shrimp per aquarium) supplied with 100 L of aerated
filtered farm water with an initial pH of 8.35, 5.0 mg L1 of dissolved oxygen, 0.50 mg L1 of total ammonia and 0.25 mg L1 of
nitrite at 28 ± 2 C for 14 days to acclimate