This work investigated the cultivation conditions for the fermentation of G. lucidum CCRC36124 in a shake-flask culture. The composition of the medium was initially screened to determine the suitable ingredients. The effects of various operational variables, including initial pH, incubation temperature and the rate of rotation, on the efficiency of fermentation were examined. The composition of the medium was then optimized by the Taguchi array method and the steepest ascent method.