Vernonia amygdalina Del. (African bitter leaf ) and Nauclea latifolia (pin cushon tree) plants were identified
and authenticated by a botanist, Department of Botany,University of Calabar. Thereafter, fresh roots of V. amygdalina were excavated and stems of the plant harvested from the Endocrine Research Farm of the University of Calabar. Also, fresh roots of N. latifolia were excavated and stem of the plant harvested from the teaching hospital premises of the university of the Calabar, while the twigs and roots of Gongronema latifolium (utazy) were purchased from Ika-Ika Qua
market in Calabar, Cross River State. The roots and stems were thoroughly washed to remove debris and the earth remains. From these the barks were divested and chopped into bits and allowed to dry under shade. The twig and root of G. latifolium were equally washed and chopped into bits and allowed to dry under shade. The dried samples were differently blended into fine powder using a Q-link electric blender Model QBL-18L40, and stored in air-tight containers.