This article presents electrophoretic depositions of chitosan(CS), bioactive glass(BG),and hydroxyapatite(HA)applied on titanium(Ti) substrate from ethanol-based suspension. The strategy was utilized to modify the deposition of particles with different values of sizes and Zeta potentials, and consequently obtain desirable nanocomposite coating. SEM studies showed that uniform distribution of particles was obtained on isolated substrate at pH4.5. Moreover, modified deposition of BG microparticles(o37 mm) and HA nanoparticles(o150 nm)in chitosan matrix was reached by changing voltage and deposition time.