medium at 50°C and poured onto Petri dishes (9 cm diameter). A 0.5 cm-PDA disk containing mycelium from each isolate was deposited in the center of each plate (Melo et al., 2013). The negative control was oil-free. Then, plates were randomized and incubated in a biochemical oxygen demand (BOD) growth chamber at 28°C and 12:12 h photoperiod. The bioassay was completely randomized with seven replications for each concentration. Thereafter, the number of the sclerotia were counted in each treatment every 24 h for 15 days.