Phytotoxin productivity
Phytotoxin assay was performed as described previously
with some modification (Iiyama et al. 1995). Each strain
was grown on PSA at 37 C for 1 day. Bacterial cells were
suspended in sterile distilled water at an OD610 of 0.2 (ca.
108 cfu/mL). Forty microliters of the cell suspension was
added to 4 mL of LB broth and incubated for 12, 16, 20,
24 h with shaking at 30 or 37 C. For extracting the toxin,
an equal volume of chloroform was added to 1 mL of
the supernatant from the liquid culture centrifuged at
10,000 rpm for 5 min. The chloroform was then rotoevaporated
from each extract, and the residue was resuspended
in 1 mL of aqueous 80 % methanol. The
absorbance of each sample was measured at 260 nm to
determine the relative amount of toxin. The chloroform
extract of LB broth alone was used as a control
Phytotoxin productivityPhytotoxin assay was performed as described previouslywith some modification (Iiyama et al. 1995). Each strainwas grown on PSA at 37 C for 1 day. Bacterial cells weresuspended in sterile distilled water at an OD610 of 0.2 (ca.108 cfu/mL). Forty microliters of the cell suspension wasadded to 4 mL of LB broth and incubated for 12, 16, 20,24 h with shaking at 30 or 37 C. For extracting the toxin,an equal volume of chloroform was added to 1 mL ofthe supernatant from the liquid culture centrifuged at10,000 rpm for 5 min. The chloroform was then rotoevaporatedfrom each extract, and the residue was resuspendedin 1 mL of aqueous 80 % methanol. Theabsorbance of each sample was measured at 260 nm todetermine the relative amount of toxin. The chloroformextract of LB broth alone was used as a control
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