The incorporated CH
film with ethanolic olive leaf extracts
(OLE) and olive pomace extracts (OPE) were prepared according
to Gol, Patel, and Rao (2013) with some modifications. A 20 g L1
CH was dispersed in an aqueous solution of glacial acetic acid
(5 mL L1, v/v) at 40 C. The solution was heated and agitated
constantly for 12 h then pH was adjusted to 5.6 with 1 moL1
NaOH. Subsequently, glycerol 16 mL L1 was added as a plasticizer
(Sánchez-González et al., 2011). The OLE and OPE 10 and 20 g L1
(w/v) were added and mixed to achieve complete dispersion.
Subsequently, they were degassed, left standing for 12 h at 25 C
and centrifuged at 8000g for 10 min. The solutions were then
dispensed on polystyrene plates and left to dry for 24 h at 25 C on
a previously leveled surface until the total evaporation of the
solvent. The dried
films were peeled from the plate and
maintained at 25 C at a relative humidity of 45%. The physical
properties of the different
films based chitosan and the biological
properties of different coating formulas based chitosan were
aimed.