Ten agrochemicals, including fungicides, insecticides, herbicides and a plant growth regulator, were quantified at
part per billion levels in complex matrices using a green-analytical chemistry (GAC) method. Liquid chromatography with dual UV/diode array (DAD) and fluorescence (FLD) detections was carried out in a single run, and the
second-order DAD-elution time and FLD-elution time data obtained were treated with MCR-ALS (multivariate
curve resolution/alternating least-squares) algorithm. In this way, while analytes are measured through their
more appropriate (absorbance and/or fluorescence) signals, chemometric treatment of the corresponding matrices allows the resolution of total or partial overlapped bands, and to overcome the presence of interferences in
real samples. In this work, FLD-elution time second-order data were obtained for the first time at two excitation
wavelengths, improving the sensitivity of fluorescent analytes. The approach was successfully applied to in land
cultivated vegetables, including mushroom, lettuce, alfalfa sprout, cucumber, and celery.