The seven cultivars of whole grain rice were washed with
distilled water three times. They were then soaked in
258 World J Microbiol Biotechnol (2010) 26:257–263
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distilled water for 72 h, while the water was changed every
24 h and finally the water was decanted. Whole grain rice
was allowed to germinate on moist paper in separate plastic
boxes at 30C. The germination process was completed by
germination of the seeds under these conditions, and germinated
rice samples were collected every 3 days during
germination. The rice germ was removed by cutting and
eliminating the bran layer by machine. Polished rice grains
were obtained from this procedure and were dried at 70C
for 12 h, then ground by blender (Komatsuzaki et al. 2007;
Xing et al. 2007). Ground samples were collected for
GABA extraction and further analysis.