Wastewater nal ef uent samples were collected monthly; two samples per site (a total of 48 samples), over a 12 month sampling period (September 2012–August 2013) from two wastewater treatment facilities in Amathole District Municipality, Eastern Cape, South Africa. The two facilities are located within the geographical coordinates 32°34′17′′S, 27°26′95′′E (Site 1), and 32o41′31′′S, 27o08′36′′E (Site 2), respectively. Both treatment plants use the activated sludge and drying beds technology, and disinfect their nal ef uents by chlorination before dis- charging into the receiving watersheds.