The aim of the present study was to evaluate the effects of chitosan-gelatin film incorporated with grape
seed extract (GSE) (1 and 2%) and Ziziphora clinopodioides essential oil (ZEO) (1 and 2%), separately and in
combination, on survival of Listeria monocytogenes and shelf life extension of minced trout fillet during
refrigerated storage (4 ± 1 C) over a period of 11 days. The control and wrapped fillet samples were
analyzed for chemical (PV and TVB-N), microbial (Total mesophilic and psychrotrophic bacteria, Pseudomonas
spp., P. fluorescens, Shewanella putrefaciens, lactic acid bacteria and Enterobacteriaceae family)
and sensory properties (odor, color and acceptability). The GC-MS analysis of the essential oil components
revealed that carvacrol (65.22%) and thymol (19.51%) were the most representative components of
the essential oil. In comparison with control group, fish spoilage was significantly delayed in samples
wrapped in chitosan-gelatin film containing different concentrations of GSE and/or ZEO (P < 0.05). The
lowest bacterial growth, PV and TVB-N content were obtained in fish samples wrapped in film containing
ZEO2%þGSE2%. The fillets treated with ZEO2%þGSE1% and ZEO2%þGSE2% had the best organoleptic
properties. Based on our findings, the antibacterial activity of chitosan-gelatin film incorporated with
GSE and ZEO enhanced the shelf life of fish fillet.