Conidial formulations were applied to substrates (paper or cotton
jersey) at a rate of 3 106 conidia/cm2 using an airbrush
sprayer (Anderson et al., 2011), to give an equivalent volume application
rate of 20 mL/m2. Spore formulations were applied to HP™
Color-Laser Paper or jersey knit cotton, which were then cut into
9 cm circles. Control substrates were sprayed with blank oil formulation
only. After spraying, substrates were allowed to dry at room
temperature overnight. Coverage of conidia was verified by
extracting the conidia from three replicate, 2 cm2 swatches in Isopar
M and counting the resulting conidial suspension using an improved
Neubauer hemocytometer.
applied