We used a flow rate of run in HPLC of 0.7 lL/min and 40 lL injection volume, and the detection wavelength was 254 nm for gallic acid, 280 nm for catechin and epicatechin, 325 nm for caffeic, chlorogenic and ellagic acids, and 365 nm for quercetin, isoquercitrin, quercitrin, rutin and kaempferol.