a b s t r a c t
When inhibitory effects on stylar RNase activity were investigated in the Japanese pear (Pyrus pyrifolia
Nakai), CuSO4 and ZnSO4 were significantly effective, and the inhibition was dose-dependent. Since
MgSO4 and CaCl2 did not show any inhibition, the active ions would be Cu2+ and Zn2+. Because Cu2+
separated clearly from the stylar proteins when protein–CuSO4 mixture was loaded on Sephadex G-10
column chromatography, the cation may not be combined strongly with the protein. The RNase activity
of the protein, furthermore, was almost completely recovered when the proteins were isolated from
the mixture by (NH4)2SO4 precipitation. Copper ions strongly reduced the RNase A activity, moderately
RNase S and weakly RNase T1 at 1 mM, while it did not affect the RNase B. The CuSO4 application at 2 mM
induced more than 30% fruit set following self-pollination but Na2SO4 and K2SO4 did not, suggests that
Cu2+ causes fruit set through reducing stylar RNase activity
a b s t r a c tWhen inhibitory effects on stylar RNase activity were investigated in the Japanese pear (Pyrus pyrifoliaNakai), CuSO4 and ZnSO4 were significantly effective, and the inhibition was dose-dependent. SinceMgSO4 and CaCl2 did not show any inhibition, the active ions would be Cu2+ and Zn2+. Because Cu2+separated clearly from the stylar proteins when protein–CuSO4 mixture was loaded on Sephadex G-10column chromatography, the cation may not be combined strongly with the protein. The RNase activityof the protein, furthermore, was almost completely recovered when the proteins were isolated fromthe mixture by (NH4)2SO4 precipitation. Copper ions strongly reduced the RNase A activity, moderatelyRNase S and weakly RNase T1 at 1 mM, while it did not affect the RNase B. The CuSO4 application at 2 mMinduced more than 30% fruit set following self-pollination but Na2SO4 and K2SO4 did not, suggests thatCu2+ causes fruit set through reducing stylar RNase activity
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