Samples were also examined with Confocal Laser Scanning
Microscopy to reveal the protein and oil distribution. The particle
dispersions were diluted 5 times, stained with a few drops of Nile
Blue (Sigma–Aldrich, St. Louis, MO, USA) and directly analysed by
CSLM. Nile Blue is a fluorescent dye suitable to monitor both the
protein and the oil distribution in the sample (Sag˘lam et al.,
2011). Samples were excited at 488 nm and measured at 520–
602 nm to detect the oil signal,
Samples were also examined with Confocal Laser ScanningMicroscopy to reveal the protein and oil distribution. The particledispersions were diluted 5 times, stained with a few drops of NileBlue (Sigma–Aldrich, St. Louis, MO, USA) and directly analysed byCSLM. Nile Blue is a fluorescent dye suitable to monitor both theprotein and the oil distribution in the sample (Sag˘lam et al.,2011). Samples were excited at 488 nm and measured at 520–602 nm to detect the oil signal,
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