Two mouse hybridomas, secreting N2-specific mAbs 1D1 and
1G8, were generated through the fusion of mouse myeloma Sp2/
0 cells with splenocytes from a mouse immunized with
X1H9N2 virus. 1D1 is IgG2a isotype, while 1G8 is IgG3. The
specificity of these two mAbs for N2 was confirmed with IFA: the
two mAbs recognized MDCK cells infected with X1 virus, or
H1N2 virus (a reassortant virus that carries the NA gene of X1 virus
and the remaining genes of H1N1 virus PR8)