tA precooled aqueous solution of 7 wt% NaOH/12 wt% urea was used to dissolve cellulose up to a con-centration of 2 wt%, which was then coagulated in an acetone/water mixture to regenerate cellulosefilm. The volume ratio of acetone to water (ϕ) had a dominant influence on film dimensional stability,film-forming ability, micromorphology, and mechanical strength. The film regenerated at ϕ = 2.0 showedexcellent performance in both dimensional stability and film-forming ability. Compared to that frompure acetone, the cellulose film from the acetone/water mixture with ϕ = 2.0 was more densely inter-woven, since the cellulosic fibrils formed during regeneration had pores with smaller average diameter.The alkali capsulated in the film during film formation could be released at quite a slow rate into thesurrounding aqueous solution. The regenerated cellulose film with adjustable structure and propertiesmay have potential applications in drug release and ultra filtration.