Therefore, adhesion
of cells to corn starch was mediated by the fusion protein. L. casei cells aggregated with corn starch,
whereas the free cells of L. casei did not form any aggregates with corn starch. Because starch adhesion will in-
crease the density of cells entrapped in starch granules, a
lower number of cells may leak out of the porous starch
granules during the preparation of the bacterial core and amy-
lose coating.
an increase in the initial cell load
of probiotic products can result in an increase in bacterial
survival during exposure to GIT conditions
The anchoring domains are usually cell-surface pro-
teins or their fragments, which are associated with the cell
surface either covalently or noncovalently the adhesion of bacteria to starch
and the bulking capacity of starch, which may markedly
modify the acidic pH, were considered as possible explana-
tions for these observations
the bacteria
are entrapped between starch granules to use the protective
effect of starch for maintaining the cell viability under ad-
verse conditions.
the fusion protein was able to medi-
ate the adhesion of cells to starch granules.
free cells were
mixed with corn starch, their survival percentage was 3.0%
higher than that of the free cells, which indicated the protec-
tive effect of corn starch on cell survival.
the entrapment of bacteria between starch granules with
the aid of CPH-SBD (the aggregation of bacteria with corn
starch) enhanced the protective effect of corn starch, and sur-
vival percentage was increased to 10.8%