This study was conducted to determine the effects of supplementation of different levels boron and copper on performance, lipids in yolk and plasma contains in layer diets. In this trial a total of 320 layer chicken (26 weeks old age) was randomly distributed in 16 experimental groups. In each experiment group were consist at 5 replicates, and in each replicates there were 4 birds. During the 16 weeks experiment period, birds were fed with 16 experimental diets. Experimental diets were consisting of all possible combination of 4 levels of added boron (0, 60, 120 and 240 mg/kg) and 4 levels of added copper (0, 75, 150 and 300 mg/kg) to the basal ration.