Variation of PCR conditions: (a) Concentration of MgCl2 was varied from 1 mM to 10 mM. (b) Thermo-cycling parameters were changed from those optimized and mentioned above by varying annealing temperature 3 8C and cycle number by 2.
Variation of PCR conditions: (a) Concentration of MgCl2 wasvaried from 1 mM to 10 mM. (b) Thermo-cycling parameterswere changed from those optimized and mentioned above byvarying annealing temperature 3 8C and cycle number by 2.