with slight
modification. Briefly, 200 l 3.75 mM 1,10-phenanthroline solution,
200 l 3.75 mM FeSO4 and 400 l 0.05% v/v H2O2 were added to
test tube containing 400 l serial dilution of (25 to 200 g/ml) of
CJSPE prepared in pH 7.4 phosphate buffer and mixed well.
Mixture was then incubated for 1 h at 37°C and absorbance was
measured at 532 nm.