2.4 Fat particle size analysis
Fat globule size distributions of ice cream mix (4 °C) and melted ice cream after
hardening (4 °C for 3–4 h) were measured by laser diffraction (Malvern Mastersizer
2000, Malvern Instruments Ltd., Malvern, Worcestershire, UK). Dilution of the
samples in the Mastersizer chamber was approximately 1:1,000 with Milli-Q. The
Basil seed gum as an ice cream stabilizer 275
refractive index for the fat and the dispersing medium was 1.46 and 1.33, respectively,
with absorbance of 0.001. Mean particle size diameter, d4,3 (the volume/surfaceweighted
diameter), was recorded (Mendez-Velasco and Goff 2011).