Materials and methods
Materials
Polished dhals (split pulses) of pigeon pea
(Cajanus cajan), green gram (Vigna radiata) and
black gram (Vigna mungo) were purchased from local
markets of Pune. Flours of dhals were prepared by
using blender. Enzymes such as α-amylase, pepsin,amyloglucosidase and pullulanase were procured
from Sigma-Aldrich. All chemicals used were of
analytical grade.