Rice powder (2.5 g) was accurately weighed and placed into an extraction vessel.
According to the experimental design,
a set volume and type of solvent was added to the extraction vessel
and the extraction was performed under different MAE conditions.
After extraction, the vessels were allowed to cool down in an ice bath for 10 min
and carefully opened in a fume cupboard.
Solid material from the sample was separated from liquid,
using a paper filter. The solid material was then washed using new solvent.
The liquid must be dried under vacuum condition by a rotary evaporator,
then re-dissolved with methanol into 2 mL
and filtered using 0.45 mm cellulose filter (Millipore) prior to injection on the HPLCeFD system.