The concentration of four monosaccharides and five inhibitors were measured before and after cultivation to evaluate the consumption of sugars and changes in the concentrations of fermentation inhibitors during cultivation. The concentration of four monosaccharides and five inhibitors were also measured for hot water extraction before and after autoclaving. The analysis of monosaccharides is summarized in Table 2. The cultivations in wood hot water extract (HWE) without autoclaving did not resultin any substantial cellulose activity and this result was some what surprising, since it is not clear that the media contained compounds that inhibit A. xylinus 23769. This could have occurred dueto contamination of the hot water extract, which was not sterilized. However,in the Uraki, Morito, Kishimoto, and Sano (2002) study, bacterial cellulose production was not observed in the unpurified water soluble fraction (WS) of waste liquor from atmospheric acetic acidpulping due to presence of antibacterial activity in the unpurified water soluble fraction. Bacterial cellulose production was only observed after purifying the mediumwith activated carbon. Therefore, wood hot water extract should also be studied in terms of antibacterial activity. Monosaccharide concentration also did not change significantly before and after autoclaving. Cultures at pH 5 and 26 and 30◦C and at pH 6 and 26◦C did not produce BC and the reason why BC is not producedat these conditions requires further investigation. The results in Table 2 indicate that glucose and xylose were the main nutrientsources in all cultivations.