The raw material (Mango Ataulfo (Mangifera indica L.)), which was employed for this work was dried
with a solar dehydrator and milled. After the pretreatment, we conducted a thermal hydrolysis process
(THP) at conditions of 130 °C and 15 psi for 20 min. After the pretreatment, it was centrifuged at 5000
rpm and 28 °C for 10 minutes (Centrifuge 5804R, 15 amp version) to clarify the substrate. The substrate
was adjusted to a concentrations of reducing sugar of 100, 150 and 200 g/L, respectively according to the
proposed experimental design.