Increasing texture values of gels prepared with NFB addition and setting was thought primarily related to endogenous TGase and calcium ion from NFB. NFB calcium ion affected the activity of endogenous TGase (Fig. 2). The formation of myosin heavy chain (MHC) crosslinks through 3-(g-glutamyl) lysine covalent bond after setting with NFB was confirmed by our previous study (Yin & Park, 2014).