• Flow-analysis (for operation see Section 10.3)
The manifold in Fig. 10-6 requires a sample time of about 2.5 min (4 ml sample) to provide a steady-state peak. Heating of the 24 turns reaction coil to 37 ˙c accelerates the reaction independent of the environmental temperature. This allows reduction of the reaction coil to 19 turns or the sample time to about 2 min (3.2 ml sample volume).
Using microtube systems, all pump tubes may be reduced to half their flow rates. The air injection has to be adjusted to provide sufficient liquid seqment volumes for the spectrophotometric operation.