Reaction mixture was incubated for 1 h at 37 C with shaking.
Afterwards, pH of sample was first adjusted with 0.1 M NaHCO3
to pH 5 and then with 0.1 M NaOH to pH 7–8. Next, pancreatin
enzyme dissolved in 0.1 M PB (pH 8) was added at an enzymeto-
sample ratio of 1:25