This study demonstrated that, Lemongrass essential oil signifcantly inhibited mycelial growth and spore germination of C. musae, F. incarnatum and F. verticillioides, pathogens associated with banana crown rot. This could be explained by synergistic efect of complex components contained in oil. This oil could be used as natural fungicide to control banana crown rot disease. However, their efcacy in vivo must be proved.