3. Results
3.1. Effect of enterocin AS-48 in samples from canned foods
and coconut milk inoculated with vegetative cells of
G. stearothermophilus
G. stearothermophilus
vegetative cells multiplied rapidly both in
the samples of canned corn and canned peas, reaching high cell
concentrations (ca. 8 log CFU/g) within the first day of incubation.
This was followed by a progressive decay of the viable population
during the subsequent 30-days incubation (
Fig. 1
A, B). In the can-
ned corn samples, a bacteriocin concentration of 1.75
m
g/g caused
some growth inhibition during the first day of incubation and
reduced the viable cell concentration below detection levels by day
3(
Fig. 1
A). A higher bacteriocin concentration (3.5
m
g/g) signifi-
cantly reduced (
P
<
0.05) the viable cell concentrations by 1.4 log
units at day 1, while 7.0
m
g/g completely inactivated all detectable
cells. Remarkably, no viable cells were detected in the bacteriocin-
treated samples during further storage for up to 30 days. Results
obtained in samples from canned peas were very similar, with
reductions of viable cell counts from 1.5 to 2.1 log units or
a complete inactivation of the bacilli within the first day for
bacteriocin concentrations of 1.75, 3.0, and 7.0
m
g/g respectively
(
Fig. 1
B).
In coconut milk,
G. stearothermophilus
showed a much lower
capacity to grow, reaching 5.4 log CFU/ml at day 1 (
Fig. 1
C).
However, some viable cells (1.3 log CFU/ml) were still detected at
day 15 of storage. At the lowest bacteriocin concentration tested
(1.75
m
g/ml), enterocin AS-48 reduced viable cell counts below
detection levels within the first 24 h of storage (
Fig. 1
C)
3. Results
3.1. Effect of enterocin AS-48 in samples from canned foods
and coconut milk inoculated with vegetative cells of
G. stearothermophilus
G. stearothermophilus
vegetative cells multiplied rapidly both in
the samples of canned corn and canned peas, reaching high cell
concentrations (ca. 8 log CFU/g) within the first day of incubation.
This was followed by a progressive decay of the viable population
during the subsequent 30-days incubation (
Fig. 1
A, B). In the can-
ned corn samples, a bacteriocin concentration of 1.75
m
g/g caused
some growth inhibition during the first day of incubation and
reduced the viable cell concentration below detection levels by day
3(
Fig. 1
A). A higher bacteriocin concentration (3.5
m
g/g) signifi-
cantly reduced (
P
<
0.05) the viable cell concentrations by 1.4 log
units at day 1, while 7.0
m
g/g completely inactivated all detectable
cells. Remarkably, no viable cells were detected in the bacteriocin-
treated samples during further storage for up to 30 days. Results
obtained in samples from canned peas were very similar, with
reductions of viable cell counts from 1.5 to 2.1 log units or
a complete inactivation of the bacilli within the first day for
bacteriocin concentrations of 1.75, 3.0, and 7.0
m
g/g respectively
(
Fig. 1
B).
In coconut milk,
G. stearothermophilus
showed a much lower
capacity to grow, reaching 5.4 log CFU/ml at day 1 (
Fig. 1
C).
However, some viable cells (1.3 log CFU/ml) were still detected at
day 15 of storage. At the lowest bacteriocin concentration tested
(1.75
m
g/ml), enterocin AS-48 reduced viable cell counts below
detection levels within the first 24 h of storage (
Fig. 1
C)
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