Vitamins
A, E were quantitated by utilizing absorption spectra
of 326 and 296 nm, respectively. In this process, methanol:acetonitril:chloroform
(47:42:11, v/v) mixture was used
as mobile phase at flow rate of 1 ml min1 in Techsphere
ODS-2 packed (5 lm particle and 80 A˚ pore size) column